- Product Name: Recombinant Core Streptavidin 4, r-cSA4
- Catalog Number: NRPA36S
- Packaging Details: 1mg, 10mg, 100mg, 500mg
- Formulation: lyophilized from 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4)
- Mol. Weight: 13.2 kDa subunit monomer; 52.8 kDa tetramer
- Pi theory: 5.14
- Theoretical activity: 18.1 U / mg protein (rc-SA: Biotin = 1: 4 (mol: mol))
- Activity: ≥15 U / mg protein （Green Enhanced Method）
- Purity: ≥95% （SDS-PAGE）
- Determination of concentration: 280nm absorption method, molar absorption coefficient is
4.74 × 104 (mol / L) -1 cm-1 c (g / L) = A280nm / 3.18 - Endotoxin: ≤5EU / mg
- Resources: Escherichia coli (E. coli)
- Storage condition: -20 oC
- Storage duration: 3 years
Streptavidin (SA) has a strong affinity for biotin. Active SA usually exists as a tetramer and the Kd constant affinity can reach 10-15 M. Since sa does not contain a glycosyl group, SA has a lower non-specific binding level than avidin on detection and application. It has been widely used in enzyme-linked immunosorbent assays, immunohistochemistry, time-resolved immunofluorescence (TRFI), quantitative PCR, single-stranded DNA preparation, purification, and preparation of biological components. monoclonal antibodies.
In theory, an SA can bind four biotinylated macromolecules, but due to the spatial barrier in biotinylated macromolecules, SA generally cannot bind 1-2 biotinylated macromolecules. We have optimized the spatial structure of streptavidin 4 at the molecular level, leading to advantages in binding with biotinylated macromolecules. Our designed product can bind 2-4 biotinylated macromolecules, and its stability is also higher than that of a common SA or SA nucleus. It is suitable for scientific research, diagnostic testing, and other fields.