HIV-1 epidemic in Belgium mainly driven by the MSM. In these patients subtype B predominates but the presence of an increase in population of non-B subtypes have been reported. We aimed to determine the extent to which the increase in the heterogeneity of subtypes in high risk populations induces the formation and deployment of a new recombinant form. This study focused on the transmission network which reflects the local transmission to the important ones.
One hundred and five HIV-1 transmission clusters were identified after 2849 phylogenetic analysis of HIV-1 pol sequences generated for the purpose of drug resistance test basis between 2013 and 2017. Of this group 105, 62 extended in size over the last two years and therefore considered as representing continuous transmission. These 62 clusters including 774 patients in total. From each cluster between 1 and 3 patient representatives have been selected to close the full-length viral genome sequencing. In total, 101 patients full genome sequence generated. Indications for the presence of recombinant forms newfound to 10 clusters.
Food DNA Extraction Kit (Proteinase K included) |
FE05 |
GeneOn |
5 preps |
Ask for price |
DiscoveryProbe? DNA Damage/DNA Repair Library |
L1033-.1 |
ApexBio |
100 uL/well(10 mM solution) |
EUR 4666.8 |
DiscoveryProbe? DNA Damage/DNA Repair Library |
L1033-.25 |
ApexBio |
250 uL/well(10 mM solution) |
EUR 8286 |
DiscoveryProbe? DNA Damage/DNA Repair Library |
L1033-5 |
ApexBio |
5 mg/well |
EUR 10806 |
T7 DNA |
310-005 |
GeneOn |
200µg |
EUR 98.4 |
T7 DNA |
310-025 |
GeneOn |
5x200 µg |
EUR 270 |
pUC18 DNA |
G40-300 |
GeneOn |
50µg |
EUR 67.2 |
pUC18 DNA |
G40-305 |
GeneOn |
5x50µg |
EUR 176.4 |
DNA Ligase I (DNA Ligase I) Antibody |
abx232431-100ug |
Abbexa |
100 ug |
EUR 577.2 |
|
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free) |
N150 |
GeneOn |
500 units |
EUR 122.4 |
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free) |
N152 |
GeneOn |
5x500 units |
EUR 472.8 |
DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free) |
N154 |
GeneOn |
20x500 units |
EUR 1830 |
DNA antibody |
10-D20A |
Fitzgerald |
500 ug |
EUR 591.6 |
Description: Mouse monoclonal DNA antibody |
DNA antibody |
10C-CR6034M1 |
Fitzgerald |
100 ug |
EUR 289.2 |
Description: Mouse monoclonal DNA antibody |
DNA antibody |
10R-D114b |
Fitzgerald |
100 ug |
EUR 672 |
Description: Mouse monoclonal DNA antibody |
DNA Antibody |
abx023805-02mg |
Abbexa |
0.2 mg |
EUR 777.6 |
|
Activated DNA |
80605 |
BPS Bioscience |
500 µL |
EUR 125 |
Description: Activated DNA for PARP assay, for use with BPS's assay kits for PARPs 1, 2, 3, and 6. PARP1 Chemiluminescent Assay Kit, #80551 ; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553; PARP6 Chemiluminescent Assay Kit, #80556; PARP2 Homogeneous Assay Kit, #80702 |
DNA-Fect293 |
DF37293-1 |
Neuromics |
1 ml |
EUR 349.2 |
DNA-fectamine |
DNAF001 |
Bio Basic |
1ml |
EUR 334.57 |
|
Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled |
AD-146-U |
Alpha Diagnostics |
Custom |
Ask for price |
Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated |
AD-146-B |
Alpha Diagnostics |
Custom |
Ask for price |
Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase) |
TG2000G-7KIT |
TopoGen |
2000 assays |
EUR 3412.8 |
Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase) |
TG2000G-5KIT |
TopoGen |
1000 assays |
EUR 1934.4 |
Human DNA-directed DNA/RNA polymerase mu (POLM) |
1-CSB-BP889065HU |
Cusabio |
-
EUR 1008.00
-
EUR 398.40
-
EUR 2604.00
-
EUR 1362.00
-
EUR 1894.80
-
EUR 564.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus |
Human DNA-directed DNA/RNA polymerase mu (POLM) |
1-CSB-YP889065HU |
Cusabio |
-
EUR 703.20
-
EUR 358.80
-
EUR 2606.40
-
EUR 1080.00
-
EUR 1730.40
-
EUR 458.40
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast |
Human DNA-directed DNA/RNA polymerase mu (POLM) |
1-CSB-EP889065HU |
Cusabio |
-
EUR 606.00
-
EUR 318.00
-
EUR 2192.40
-
EUR 919.20
-
EUR 1461.60
-
EUR 402.00
|
- 100ug
- 10ug
- 1MG
- 200ug
- 500ug
- 50ug
|
|
Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli |
Maximo DFS-Plus Taq DNA Polymerase DNA-free |
N140 |
GeneOn |
500 units |
EUR 60 |
Maximo DFS-Plus Taq DNA Polymerase DNA-free |
N142 |
GeneOn |
5x500 units |
EUR 144 |
Maximo DFS-Plus Taq DNA Polymerase DNA-free |
N144 |
GeneOn |
20x500 units |
EUR 440.4 |
Taq DNA polymerase (TQ21) , DNA Aptamer, FITC labeled |
AD-146-F |
Alpha Diagnostics |
Custom |
Ask for price |
Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase) |
TG2000G-1KIT |
TopoGen |
100 assays |
EUR 456 |
Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase) |
TG2000G-3KIT |
TopoGen |
500 assays |
EUR 1182 |
Human Neutrophil Elastase (DNA I) , DNA Aptamer, unlabeled |
AD-155-U |
Alpha Diagnostics |
Custom |
Ask for price |
Recombinant human DNA-directed DNA/RNA polymerase mu |
P2815 |
FN Test |
100ug |
Ask for price |
|
Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu |
Human Neutrophil Elastase (DNA I) , DNA Aptamer, Biotinylated |
AD-155-B |
Alpha Diagnostics |
Custom |
Ask for price |
Mouse Anti-DNA Antibody (DNA) ELISA Kit |
abx257816-96tests |
Abbexa |
96 tests |
EUR 895.2 |
|
2K DNA Marker |
20-abx098045 |
Abbexa |
|
|
|
5K DNA Marker |
20-abx098048 |
Abbexa |
|
|
|
T4 DNA Ligase |
abx098136-10kU |
Abbexa |
10 kU |
EUR 385.2 |
|
T4 DNA Ligase |
abx098136-20Ku |
Abbexa |
20 Ku |
EUR 477.6 |
|
Phage λ-DNA |
301-025 |
GeneOn |
500 µg |
EUR 122.4 |
Phage λ-DNA |
301-100 |
GeneOn |
5x500µg |
EUR 426 |
T4 DNA ligase |
402-002 |
GeneOn |
400 WEISS UNITS |
EUR 75.6 |
T4 DNA ligase |
402-010 |
GeneOn |
2000 WEISS UNITS |
EUR 184.8 |
DNA Assay Kit |
6023 |
Chondrex |
1 kit |
EUR 216.6 |
Description: DNA Assay Kit |
DNA Assay Kit |
Z5030023 |
Biochain |
250 assays |
EUR 636 |
DiscoveryProbe? DNA Damage/DNA Repair-related Compounds Panel |
L1007-5 |
ApexBio |
5 mg/well |
EUR 7172.4 |
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc. |
15K DNA Marker |
20-abx098049 |
Abbexa |
|
|
|
1Kb DNA Marker |
20-abx098050 |
Abbexa |
|
|
|
1Kb DNA Marker |
D1101-050 |
GenDepot |
50㎍ |
EUR 157.2 |
1Kb DNA Marker |
D1101-250 |
GenDepot |
5X50㎍ |
EUR 360 |
Genomic DNA Kit |
20-abx098076 |
Abbexa |
|
|
|
DNA PK Antibody |
abx149863-100ug |
Abbexa |
100 ug |
EUR 526.8 |
|
DNA PK antibody |
20R-1466 |
Fitzgerald |
100 ug |
EUR 807.6 |
Description: Rabbit polyclonal DNA PK antibody |
DNA Loading Dye |
G030 |
ABM |
6X; 3 x 1.0 ml |
EUR 94.8 |
100bp DNA Marker |
20-abx098052 |
Abbexa |
|
|
|
100bp DNA ladder |
9K-004-0002 |
Bio Basic |
0.5ml |
EUR 260.98 |
|
100bp DNA Marker |
D1100-050 |
GenDepot |
50㎍ |
EUR 198 |
100bp DNA Marker |
D1100-250 |
GenDepot |
5X50㎍ |
EUR 660 |
DNA PKcs antibody |
10R-10729 |
Fitzgerald |
100 ug |
EUR 457.2 |
Description: Mouse monoclonal DNA PKcs antibody |
DNA PKcs antibody |
20R-2017 |
Fitzgerald |
50 ug |
EUR 337.2 |
Description: Rabbit polyclonal DNA PKcs antibody |
DNA PKcs antibody |
20R-2346 |
Fitzgerald |
50 ug |
EUR 337.2 |
Description: Rabbit polyclonal DNA PKcs antibody |
DNA-PK Antibody |
3130-100 |
Biovision |
each |
EUR 405.6 |
Human Genomic DNA |
BIO-35025 |
Bioline |
500µl @ 200ng/µl |
Ask for price |
DNA PKcs antibody |
70R-50261 |
Fitzgerald |
100 ul |
EUR 292.8 |
Description: Purified Polyclonal DNA PKcs antibody |
DNA-PK Antibody |
33358-100ul |
SAB |
100ul |
EUR 302.4 |
DNA-PK Antibody |
33358-50ul |
SAB |
50ul |
EUR 224.4 |
DNA PKcs Antibody |
48923-100ul |
SAB |
100ul |
EUR 399.6 |
DNA PKcs Antibody |
48923-50ul |
SAB |
50ul |
EUR 286.8 |
T4 DNA Polymerase |
N101-01 |
Vazyme |
2000 U |
EUR 278.4 |
T4 DNA Polymerase |
M1211-100 |
Biovision |
each |
EUR 385.2 |
DL5000 DNA Marker |
MD102-01 |
Vazyme |
250 μl |
EUR 128.4 |
DL5000 DNA Marker |
MD102-02 |
Vazyme |
500 μl |
EUR 135.6 |
DNA Loading Buffer |
abx098143-5ml |
Abbexa |
5 ml |
EUR 210 |
|
DNA polymerase ? (Rat) |
10-101 |
Sceti |
20ug |
EUR 465.6 |
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur. |
DNA polymerase ? (Rat) |
10-102 |
Sceti |
100ug |
EUR 1107.6 |
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur. |
Taq DNA Polymerase |
BA00103 |
Cusabio |
500U |
EUR 104.4 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA Polymerase |
BA00104 |
Bioatlas |
1000U |
EUR 147.6 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA Polymerase |
BA00105 |
Bioatlas |
2500U |
EUR 278.4 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Pfu DNA Polymerase |
BA00503 |
Bioatlas |
500U |
EUR 174 |
Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
Pfu DNA Polymerase |
BA00504 |
Bioatlas |
1000U |
EUR 286.8 |
Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
Pfu DNA Polymerase |
BA00505 |
Bioatlas |
2500U |
EUR 626.4 |
Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
Taq DNA polymerase |
BT10101 |
Bioatlas |
500U |
EUR 96 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA polymerase |
BT10102 |
Bioatlas |
1000U |
EUR 130.8 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA polymerase |
BT10103 |
Bioatlas |
2000U |
EUR 200.4 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA Polymerase |
9001-2500 |
Biovision |
each |
EUR 614.4 |
Taq DNA Polymerase |
9001-500 |
Biovision |
each |
EUR 229.2 |
PFU DNA Polymerase |
9003-2500 |
Biovision |
each |
EUR 614.4 |
PFU DNA Polymerase |
9003-500 |
Biovision |
each |
EUR 216 |
Laq? DNA Polymerase |
9004-2500 |
Biovision |
each |
EUR 732 |
Laq? DNA Polymerase |
9004-500 |
Biovision |
each |
EUR 261.6 |
DNA Cleared Plasma |
DCP100A-1 |
SBI |
100 ml |
EUR 417 |
DNA Sample Diluent |
D006-100 |
Cygnus Technologies |
100 ml |
EUR 447.6 |
|
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
DNA Sample Diluent |
D006-1000 |
Cygnus Technologies |
1000 ml |
EUR 1400.4 |
|
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
DNA Sample Diluent |
D006-500 |
Cygnus Technologies |
500 ml |
EUR 871.2 |
|
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Taq DNA Polymerase |
L7051001 |
Biochain |
1000 unit |
EUR 159 |
Taq DNA Polymerase |
L7051200 |
Biochain |
200 unit |
EUR 41 |
DL15000 DNA Marker |
MD103-01 |
Vazyme |
250 μl |
EUR 130.8 |
DL15000 DNA Marker |
MD103-02 |
Vazyme |
500 μl |
EUR 138 |
Mycoplasma DNA Kit |
K1416-250 |
Biovision |
each |
EUR 673.2 |
Mycoplasma DNA Kit |
K1416-50 |
Biovision |
each |
EUR 379.2 |
Pfu DNA Polymerase |
S116 |
GeneOn |
1x250 units |
EUR 91.2 |
Pfu DNA Polymerase |
S117 |
GeneOn |
2x250 units |
EUR 112.8 |
Pfu DNA Polymerase |
S118 |
GeneOn |
10x250 units |
EUR 363.6 |
Bst DNA Polymerase |
S600 |
GeneOn |
2000 U |
EUR 128.4 |
100 bp DNA Ladder |
M1158-500 |
Biovision |
each |
EUR 333.6 |
100 bp DNA Ladder |
MD104-01 |
Vazyme |
250 μl |
EUR 132 |
100 bp DNA Ladder |
MD104-02 |
Vazyme |
500 μl |
EUR 139.2 |
DNA ligase Antibody |
20-abx110554 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Circulating DNA Kit |
abx098870-50rxns |
Abbexa |
50 rxns |
EUR 477.6 |
|
MyFi DNA Polymerase |
BIO-21117 |
Bioline |
250 Units |
Ask for price |
MyFi DNA Polymerase |
BIO-21117/S |
Bioline |
Sample |
Ask for price |
MyFi DNA Polymerase |
BIO-21118 |
Bioline |
500 Units |
Ask for price |
MyFi DNA Polymerase |
BIO-21119 |
Bioline |
2500 Units |
Ask for price |
CHO DNA Concentrate |
D552 |
Cygnus Technologies |
1 ml |
EUR 650.4 |
|
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
CHO DNA Concentrate |
D556 |
Cygnus Technologies |
120 ul |
EUR 562.8 |
|
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
Circulating DNA Kit |
abx298027-50rxns |
Abbexa |
50 rxns |
EUR 477.6 |
|
DNA-PKcs Antibody |
R31076 |
NSJ Bioreagents |
100 ug |
EUR 356.15 |
Description: Protein Kinase DNA-Activated catalytic subunit, also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al.(1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller(1992) noted that DNA-PKcs had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al.(1999) demonstrated that the DNA-PKcs protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase. |
ClearSight DNA Stain |
BH40501 |
Bioatlas |
1ml |
EUR 123.6 |
Description: For DNA staining and vizualization. |
These 10 clusters represented 105 patients or 13.6% of the patients covered by the study. The findings clearly show that the new recombinant strains that contribute to local transmission, even in epidemic largely driven MSM and subtype B. It is an evolution that needs to be monitored as a genomic fragment reshuffled through recombination can affect the transmissibility of viruses and pathology of infection. In addition, important changes in the virus genome sequence can be challenging performance tests used for diagnosis, patient monitoring and analysis of drug resistance.