Enhanced HIV-1 Replication in CD4+ T Cells Derived from Individuals with Latent Mycobacterium tuberculosis Infection.

Mycobacterium tuberculosis (Mtb) and HIV co-infection increases mortality, accelerating progression to AIDS and worsen the disease tuberculosis. However, the impact of existing Mtb infection in subsequent HIV infection has not been fully explored.

We hypothesize that Mtb infection immunology create an environment that affect course of HIV infection and to investigate whether there was an impact Mtb infection of CD4 + T cell susceptibility to HIV-1 infection.Plasma and CD4 + T cells isolated from the blood of HIV-negative spectrum Mtb infection and individual control of non-Mtb-infected analyzed for markers of inflammation and cell phenotype T cell CD4 + T-cells infected with HIV-1 in vitro and monitored for viral replication.

We observable differences in pro-inflammatory cytokines and the relative proportion of a memory T cell subsets depending on the status of Mtb infection. Cells CD4 + T-cells derived from individuals with latent Mtb infection is supported by more efficient HIV-1 transcription, release, and replication. Enhanced HIV-1 replication correlated with a higher percentage of CD4 + TEM and TTD-existing cells.Pre Mtb infection immunology create an environment that reflects the status of Mtb infection and the effect of CD4 + T cell susceptibility to HIV-1 replication. These findings provide insight into the cellular and molecular how there Mtb infection with HIV-1 pathogenesis influence.

DNA Gyrase Assay Kit
TG1003 100 assays
EUR 313

QuantiFluo DNA Assay Kit
QFDN-250 250
EUR 351
Description: Quantitative determination of DNA by fluorimetric (340nm/450nm) method. Procedure: 5 min. Kit size: 250 tests. Detection limit: 100 ng/mL. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

ApoBrdU DNA Fragmentation Assay Kit
K2070-60 60 assays
EUR 766

ApoDIRECT DNA Fragmentation Assay Kit
K2071-50 50 assays
EUR 766

ApoBrdU DNA Fragmentation Assay Kit
K401-60
EUR 773

ApoDIRECT DNA Fragmentation Assay Kit
K402-50
EUR 794

DNA Quantification Assay Kit (Fluorometric)
K539-200
EUR 262

DNA Quantification Assay Kit (Fluorometric)
K539-2000
EUR 392

ApoBrdU DNA Fragmentation Assay Kit
55R-1396 60 assays
EUR 1083
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoDIRECT DNA Fragmentation Assay Kit
55R-1397 50 assays
EUR 1086
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoBrdU-IHC DNA Fragmentation Assay Kit
K2072-50 50 assays
EUR 835

ApoBrdU-IHC DNA Fragmentation Assay Kit
K403-50
EUR 794

ApoBrdU-IHC DNA Fragmentation Assay Kit
55R-1398 50 assays
EUR 1086
Description: DNA Fragmentation Assay Kit for use in the research laboratory

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit
P-3019 96 Assays
EUR 889.55
Description: The best epigenetics products

EpiQuik In Situ DNA Damage Assay Kit
P-6001 2x96 assays
EUR 1034.55
Description: kits suitable for this type of research

Human TDP1 Assay Kit (DNA/Top1 Complex)
TG1004-1A 100 assays
EUR 560

Human TDP1 Assay Kit (DNA/Top1 Complex)
TG1004-2A 250 assays
EUR 660

Topoisomerase I Assay Kit (25ug SC DNA)
TG1015-1 100 assays
EUR 302

Topoisomerase I Assay Kit (62.5ug SC DNA)
TG1015-2 250 assays
EUR 414

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)
TG2000G-1KIT 100 assays
EUR 380

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)
TG2000G-3KIT 500 assays
EUR 985

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)
TG2000G-5KIT 1000 assays
EUR 1612

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)
TG2000G-7KIT 2000 assays
EUR 2844

EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)
P-2004 96 Assays
EUR 614.05
Description: kits suitable for this type of research

EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric)
P-2005 96 Assays
EUR 628.55
Description: Ask the seller for details

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
P-3001 96 Assays
EUR 875.05
Description: fast delivery possible

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit
P-3008 96 Assays
EUR 860.55
Description: Ask the seller for details

HIF-1 Alpha DNA Binding Activity Assay Kit
CBA-282 96 assays
EUR 635
Description: Cell Biolabs? HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.

EvaEZ Flurometric DNA Polymerase Activity Assay (200 rxn)
29051 2mL
EUR 459
Description: Minimum order quantity: 1 unit of 2mL

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)
P-3004 96 Assays
EUR 889.55
Description: The best epigenetics products

EZClick? TUNEL ? in situ DNA Fragmentation/Apoptosis Assay Kit
K191-100
EUR 533

Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)
P-3094 96 Assays
EUR 904.05
Description: fast delivery possible

Topoisomerase I Assay Kit (25ug SC DNA +100u Human Topo I)
TG1015-1A 100 assays
EUR 403

Topoisomerase I Assay Kit (62.5ug SC DNA + 250u Human Topo I)
TG1015-2A 250 assays
EUR 660

Topoisomerase I Assay Kit (125ug SC DNA + 500u Human Topo I)
TG1015-3A 500 assays
EUR 772

XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)
30007 1KIT
EUR 236
Description: Minimum order quantity: 1 unit of 1KIT

Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay)
CBA-200 50 assays
EUR 560
Description: For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels. Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesis in vitro. The ECM gel matrix very closely resembles an in vivo environment.

Lactose Assay Kit
MET-5001 100 assays
EUR 432
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe.

Bilirubin Assay Kit
MET-5010 200 assays
EUR 479
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus.

Pyruvate Assay Kit
MET-5029 100 assays
EUR 479
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.

Glycine Assay Kit
MET-5070 100 assays
EUR 450

Taurine Assay Kit
MET-5071 200 assays
EUR 508

Sarcosine Assay Kit
MET-5072 100 assays
EUR 450

Tyrosine Assay Kit
MET-5073 100 assays
EUR 450

Phospholipid Assay Kit
MET-5085 96 assays
EUR 456

Ammonia Assay Kit
MET-5086 100 assays
EUR 456

Adenosine Assay Kit
MET-5090 100 assays
EUR 508

Inosine Assay Kit
MET-5092 100 assays
EUR 508

Alanine Assay Kit
MET-5093 200 assays
EUR 508

Histamine Assay Kit
AKR-360 96 assays
EUR 519
Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve.

Glucose Assay Kit
abx090673-1Kit 1 Kit
EUR 237

ADA Assay Kit
abx090675-100tests 100 tests
EUR 237

Glutamate Assay Kit
abx096004-100Assays 100 Assays
EUR 441

Glutathione Assay Kit
abx096005-100Assays 100 Assays
EUR 378

Trehalase Assay Kit
abx096014-100Assays 100 Assays
EUR 551

Pyruvate Assay Kit
abx097982-100Assays 100 Assays
EUR 472

NADPase Assay Kit
abx097983-100Assays 100 Assays
EUR 504

Starch Assay Kit
abx097988-100Assays 100 Assays
EUR 441

Trehalose Assay Kit
abx097995-100Assays 100 Assays
EUR 472

ADA Assay Kit
abx098403-Hitachi7060R190ml2R290ml1 Hitachi 7060; R1: 90ml×2 R2: 90ml×1
EUR 739

ADA Assay Kit
abx098403-Hitachi7170R140ml4R220ml4 Hitachi 7170; R1: 40ml×4 R2: 20ml×4
EUR 801

ADA Assay Kit
abx098403-Hitachi7170R160ml4R260ml2 Hitachi 7170; R1: 60ml×4 R2: 60ml×2
EUR 911

ADA Assay Kit
abx098403-Toshiba40R150ml4R250ml2 Toshiba 40; R1: 50ml×4 R2: 50ml×2
EUR 786

Calcium Assay Kit
abx098414-Hitachi7020R140ml2R240ml2 Hitachi 7020; R1: 40ml×2 R2: 40ml×2
EUR 206

Calcium Assay Kit
abx098414-Hitachi7060R190ml1R290ml1 Hitachi 7060; R1: 90ml×1 R2: 90ml×1
EUR 206

Calcium Assay Kit
abx098414-Toshiba40R140ml2R240ml2 Toshiba 40; R1: 40ml×2 R2: 40ml×2
EUR 206

Calcium Assay Kit
abx098414-UniversalR140ml2R240ml2 Universal; R1: 40ml×2 R2: 40ml×2
EUR 206

Cholinesterase Assay Kit
abx098416-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 300

Cholinesterase Assay Kit
abx098416-Hitachi7170R140ml3R230ml1 Hitachi 7170; R1: 40ml×3 R2: 30ml×1
EUR 316

Cholinesterase Assay Kit
abx098416-Hitachi7170R160ml2R230ml1 Hitachi 7170; R1: 60ml×2 R2: 30ml×1
EUR 316

Cholinesterase Assay Kit
abx098416-Toshiba40R140ml4R240ml1 Toshiba 40; R1: 40ml×4 R2: 40ml×1
EUR 331

Cholinesterase Assay Kit
abx098416-UniversalR160ml2R215ml2 Universal; R1: 60ml×2 R2: 15ml×2
EUR 316

Creatinine Assay Kit
abx098422-Hitachi7020R150ml3R250ml1 Hitachi 7020; R1: 50ml×3 R2: 50ml×1
EUR 519

Creatinine Assay Kit
abx098422-Hitachi7060R190ml2R260ml1 Hitachi 7060; R1: 90ml×2 R2: 60ml×1
EUR 472

Creatinine Assay Kit
abx098422-Toshiba120R140ml3R240ml1 Toshiba 120; R1: 40ml×3 R2: 40ml×1
EUR 566

Creatinine Assay Kit
abx098422-Toshiba120R150ml3R250ml1 Toshiba 120; R1: 50ml×3 R2: 50ml×1
EUR 519

Creatinine Assay Kit
abx098422-Toshiba40R150ml3R250ml1 Toshiba 40; R1: 50ml×3 R2: 50ml×1
EUR 519

Fructosamine Assay Kit
abx098427-Hitachi7020R140ml2R220ml1 Hitachi 7020; R1: 40ml×2 R2: 20ml×1
EUR 253

Fructosamine Assay Kit
abx098427-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 253

Fructosamine Assay Kit
abx098427-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 206

Fructosamine Assay Kit
abx098427-Hitachi7170R140ml12R240ml3 Hitachi 7170; R1: 40ml×12 R2: 40ml×3
EUR 222

Fructosamine Assay Kit
abx098427-Hitachi7170R140ml2R220ml1 Hitachi 7170; R1: 40ml×2 R2: 20ml×1
EUR 253

Glucose Assay Kit
abx098429-Hitachi7020R140ml4R240ml1 Hitachi 7020; R1: 40ml×4 R2: 40ml×1
EUR 222

Glucose Assay Kit
abx098429-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 222

Glucose Assay Kit
abx098429-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 222

Haptoglobin Assay Kit
abx098432-BeckmanR140ml1R210ml1 Beckman; R1: 40ml×1 R2: 10ml×1
EUR 347

Haptoglobin Assay Kit
abx098432-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1: 40ml×1 R2: 10ml×1
EUR 347

Haptoglobin Assay Kit
abx098432-Hitachi7170R120ml1R25ml1 Hitachi 7170; R1: 20ml×1 R2: 5ml×1
EUR 269

Haptoglobin Assay Kit
abx098432-Toshiba40R140ml1R210ml1 Toshiba 40; R1: 40ml×1 R2: 10ml×1
EUR 347

Homocysteine Assay Kit
abx098434-Hitachi7060R160ml1R210ml1 Hitachi 7060; R1: 60ml×1 R2: 10ml×1
EUR 1036

Homocysteine Assay Kit
abx098434-Hitachi7170R130ml2R210ml1 Hitachi 7170; R1: 30ml×2 R2: 10ml×1
EUR 1036

Homocysteine Assay Kit
abx098434-Toshiba40R130ml2R210ml1 Toshiba 40; R1: 30ml×2 R2: 10ml×1
EUR 1036

Homocysteine Assay Kit
abx098434-UniversalR130ml2R210ml1 Universal; R1: 30ml×2 R2: 10ml×1
EUR 1036

Iron Assay Kit
abx098439-Hitachi7020R140ml1R210ml1 Hitachi 7020; R1:40ml×1 R2:10ml×1
EUR 206

Iron Assay Kit
abx098439-Hitachi7170R140ml1R210ml1 Hitachi 7170; R1:40ml×1 R2:10ml×1
EUR 206

Iron Assay Kit
abx098439-Hitachi7170R160ml1R215ml1 Hitachi 7170; R1:60ml×1 R2:15ml×1
EUR 222
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